Vasorelaxant alkaloids from Spirospermum penduliflorum (Menispermaceae), a plant used to treat hypertension in malagasy traditional medicine

Raoelison, Emmanuel Guy;Rafamantanana, Mamy Harisoa;Razafindrazaka, René;Randriantsoa, Adolphe;Quetin-Leclercq, Joëlle;et.al.
(2013) Natural Product Communications : an international journal for communications and reviews — Vol. 8, n° 5, p. 575-578 (2013)

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Authors
  • Raoelison, Emmanuel Guy
    Author
  • Rafamantanana, Mamy Harisoa
    Author
  • Razafindrazaka, René
    Author
  • Randriantsoa, Adolphe
    Author
  • Morel, NicoleUCLouvain
    Author
  • Quetin-Leclercq, JoëlleUCLouvain
    Author
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Abstract
Spirospermum penduliflorum Thouars (Menispermaceae) is widely used on the eastern coast of Madagascar to treat hypertension. The aim of the present study was to analyse the vasorelaxant properties of different leaf extracts. The activity of the n-hexane, dichloromethane and methanolic extracts was tested on phenylephrine-contracted aorta. The dichloromethane extract was shown to be the most effective. Further fractionation of this extract led to the isolation of an active fraction relaxing phenylephrine-contracted aorta with an IC50 of 0.18 μg/mL {log IC50 (μg/mL) -0.74 ± 0.03} but was much less effective on KCl induced contractions. Bioassay-guided fractionation of this fraction led to the isolation of two aporphinoid alkaloids, neolitsine and dicentrine, which at concentrations of 0.1 μM and 1 μM displaced to the right the phenylephrine concentration-contraction curve. Our results show that Spirospermum penduliflorum extracts possess vasorelaxant activity in vitro that could be related to the presence of dicentrine in the extracts having an alpha1 antagonist activity. This finding is not in accord with the previous studies by Rasoanaivo et al. where no alkaloids were detected in the leaves of Spirospermum penduliflorum
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Raoelison, E. G., Rafamantanana, M. H., Razafindrazaka, R., Randriantsoa, A., Urverg-Ratsimamanga, S., Morel, N., & Quetin-Leclercq, J. (2013). Vasorelaxant alkaloids from Spirospermum penduliflorum (Menispermaceae), a plant used to treat hypertension in malagasy traditional medicine. Natural Product Communications : an international journal for communications and reviews, 8(5), 575-578. https://hdl.handle.net/2078.5/201511 (Original work published 2013)