Efficient Expression of Bovine Alpha-lactalbumin in Saccharomyces-cerevisiae
(1991) European Journal of Biochemistry — Vol. 202, n° 2, p. 471-477 (1991)
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- Abstract
- A synthetic gene encoding the mature bovine alpha-lactalbumin fused to the preproregion of the yeast alpha-mating factor has been expressed and secreted at high level in Saccharomyces cerevisiae under the control of the alpha-mating promotor. Growth conditions were found to be critical for the expression: recombinant alpha-lactalbumin could only be detected in the medium provided the culture was grown at neutral pH. The secreted bovine alpha-lactalbumin is enzymatically active and identical to the whey protein, as confirmed by SDS/PAGE, IEF, ultraviolet and CD spectral analysis, and amino-terminal sequence determination.
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Viaene, A., Volckaert, G., Joniau, M., Debaetselier, A., & Vancauwelaert, F. (1991). Efficient Expression of Bovine Alpha-lactalbumin in Saccharomyces-cerevisiae. European Journal of Biochemistry, 202(2), 471-477. https://doi.org/10.1111/j.1432-1033.1991.tb16396.x (Original work published 1991)