Multicenter inter-laboratory analysis of routine susceptibility testing with a challenge panel of resistant strains.

Deckers, Corentin;Soleimani, Reza;Denis, Olivier;Bogaerts, Pierre;Belgian National Antibiogram Committee;et.al.
(2022) Journal of Global Antimicrobial Resistance — Vol. 28, p. 125-129 (2022)

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Abstract
(en) OBJECTIVES: In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. METHODS: The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method. RESULTS: Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics. CONCLUSION: Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.
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Deckers, C., Soleimani, R., Denis, O., Bogaerts, P., Berhin, C., Rodriguez-Villalobos, H., Descy, J., Hallin, M., Nonhoff, C., Desmet, S., Magerman, K., Vanden Abeele, A. M., Lissoir, B., Matheeussen, V., Vernelen, K., & Huang, T.-D. (2022). Multicenter inter-laboratory analysis of routine susceptibility testing with a challenge panel of resistant strains. Journal of Global Antimicrobial Resistance, 28, 125-129. https://doi.org/10.1016/j.jgar.2021.12.020 (Original work published 2022)