Evaluation of a Rigid Fibrin Matrix (F50/T50) for the Survival of Human Isolated Follicles after 2 and 7 Days of Xenografting
(2015)
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- et. al.
- Abstract
- Objective: Fibrin is a promising scaffold for the artificial ovary. Recently, a matrix with low fibrinogen and thrombin concentrations (12.5 mg/ml and 1 UI/ml respectively) tested for encapsulation of murine follicles showed ∼30% follicle recovery rate 1 week after autotransplantation. Results for human follicles in this matrix are however deceiving. Human ovarian cortex being more rigid than the mouse ovary, human follicles may require a stiffer matrix for their survival. The study was designed to test this hypothesis. Methods: Fresh human ovarian tissue was enzymatically digested and follicles isolated under stereomicroscope. Around 50 follicles were encapsulated in a more rigid fibrin matrix (50 mg/ml fibrinogen and 50 UI/ml thrombin) and grafted to ovarian bursa of 9 nude mice. Mice were euthanized after 10 minutes (control), 2 days and 7 days, and recovered grafts analyzed at histology (every 10 μm) for the presence of follicles. Only healthy looking follicles with an oocyte were counted. Results: All grafts (3 for each time period) were recovered. Just 48% of the follicles initially embedded in the matrix were found in the control group (10 min). At day 2 and day 7, the follicle recovery rate was 28% and 23.4% respectively. Discussion: A non-negligible proportion of follicles are lost during clot formation and grafting. However, >50% of remaining follicles survive after 2 and 7 days. This is the first time isolated human follicles are shown to survive in fibrin in vivo and confirms the need for a rigid matrix. These results are very encouraging for the construction of the artificial ovary.
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Soares M., Chiti M, Paulini F, & et al. (2015). Evaluation of a Rigid Fibrin Matrix (F50/T50) for the Survival of Human Isolated Follicles after 2 and 7 Days of Xenografting. https://hdl.handle.net/2078.5/92060