Non-invasive hydrodynamic imaging in plant roots at cellular resolution

Pascut, Flavius C.;Couvreur, Valentin;Dietrich, Daniela;Leftley, Nicky;Webb, Kevin F.;et.al.
(2021) Nature Communications — Vol. 12, n° 1, p. 7 (2021)

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Authors
  • Pascut, Flavius C.orcid-logoOptics & Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham, UK
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  • Couvreur, Valentinorcid-logoUCLouvain
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  • Dietrich, Danielaorcid-logoSchool of Biological Sciences, University of Bristol, Bristol, UK
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  • Leftley, NickyDivision of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington, UK
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  • Draye, Xavierorcid-logoUCLouvain
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  • Webb, Kevin F.orcid-logoOptics & Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham, UK
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Abstract
A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’.
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Pascut, F. C., Couvreur, V., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., Calvo-Polanco, M., Casimiro, I., Maurel, C., Salt, D. E., Draye, X., Wells, D. M., Bennett, M. J., & Webb, K. F. (2021). Non-invasive hydrodynamic imaging in plant roots at cellular resolution. Nature Communications, 12(1), 7. https://doi.org/10.1038/s41467-021-24913-z (Original work published 2021)