Random mutant generation and its utility in uncovering structural and functional features of cytochrome b in Saccharomyces cerevisiae.
(1993) Journal of Bioenergetics and Biomembranes — Vol. 25, n° 3, p. 211-220 (1993)
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- The generation of random mutations in the mitochondrial cytochrome b gene of Saccharomyces cerevisiae has been used as a most fruitful means of identifying subregions that play a key role in the bc1 complex mechanism, best explained by the protonmotive Q cycle originally proposed by Peter Mitchell. Selection for center i and center o inhibitor resistance mutants, in particular, has yielded much information. The combined approaches of genetics and structural predictions have led to a two-dimensional folding model for cytochrome b that is most compatible with current knowledge of the protonmotive Q cycle. A three-dimensional model is emerging from studies of distant reversions of deficient mutants. Finally, interactions between cytochrome b and the other subunits of the bc1 complex, such as the iron-sulfur protein, can be affected by a single amino acid change.
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Colson, A. M. (1993). Random mutant generation and its utility in uncovering structural and functional features of cytochrome b in Saccharomyces cerevisiae. Journal of Bioenergetics and Biomembranes, 25(3), 211-220. https://doi.org/10.1007/BF00762583 (Original work published 1993)