Engineering Nicotiana tabacum trichomes for triterpenic acid production
(2023) Plant Science — Vol. 328, p. 111573 (2023)
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- In this work, we aimed at implementing the biosynthesis o triterpenic acids in Nicotiana tabacum glandular trichomes. Although endogenous genes coding or enzymes involved in such biosynthetic pathway are ound in the Nicotiana tabacum genome, implementing such pathway specically in glandular trichomes required to boost endogenous enzymatic activities. Five transgenes coding or a arnesyl-diphosphate synthase, a squalene synthase, a squalene epoxidase, a beta-amyrin synthase and a beta-amyrin 28-monooxygenase were introduced in N. tabacum, their expression being driven by pMALD1, a trichome-specic transcriptional promoter. This study aimed at testing whether sinking isoprenoid precursors localized in plastids, by exploiting potential cross-talks allowing the exchange o terpenoid pools rom the chloroplast to the cytosol, could be a way to improve overall yield. By analyzing metabolites extracted rom entire leaves, a low amount o ursolic acid was detected in plants expressing the ve transgenes. Our study shows that the terpene biosynthetic pathway could be, in part, redirected in N.tabacum glandular trichomes with no deleterious phenotype at the whole plant level (chlorosis, dwarsm,…). In light o our results, possible ways to improve the nal yield are discussed.
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Gossart, N., Berhin, A., Sergeant, K., Alam, I., André, C., Hausman, J.-F., Boutry, M., & Hachez, C. (2023). Engineering Nicotiana tabacum trichomes for triterpenic acid production. Plant Science, 328, 111573. https://doi.org/10.1016/j.plantsci.2022.111573 (Original work published 2023)