Metallic-based phthalocyanine nanoemulsions for photodynamic purging of ovarian tissue in leukemia patients.
(2025) Colloids and Surfaces B: Biointerfaces — Vol. 245, p. 114338 (2025)
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- For cancer patients with a high risk of ovarian tissue metastasis, ovarian autotransplantation is not advised due to the potential spread of malignant cells. Ex vivo purging of ovarian fragments may offer a more suitable alternative for fertility restoration. Eradicating malignant cells should be done selectively without affecting follicles or ovarian stromal cells (SCs). As a clinically licensed method, photodynamic therapy (PDT) is a minimally invasive treatment to destroy cancer cells. This study evaluates the effectiveness of nanoemulsions (NE) containing two phthalocyanine photosensitizers; aluminum (III) phthalocyanine (AlPc) and zinc (II) phthalocyanine (ZnPc) in eliminating cancer cells. Human leukemic malignant (HL-60) and ovarian stromal cells (SCs) were treated with AlPc/ZnPc loaded NEs with or without diode laser irradiation. HL-60 leukemia cells in 2D culture were eliminated by treatment with 10 nM AlPc-NE or 0.1 µM ZnPc-NE, while no toxicity was observed in SCs. In 3D culture models, although the cells showed more resistance to the NEs as a result of limited oxygen and photosensitizer penetration, the treatment remained selective for cancer cells. These approaches have the potential to eliminate malignant cells from ovarian tissue fragments.
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Moghassemi, S., Dadashzadeh, A., Nikanfar, S., Ghaffari-Bohlouli, P., de Souza, P. E. N., Shavandi, A., Azevedo, R. B. d., & Andrade Amorim, C. (2025). Metallic-based phthalocyanine nanoemulsions for photodynamic purging of ovarian tissue in leukemia patients. Colloids and Surfaces B: Biointerfaces, 245, 114338. https://doi.org/10.1016/j.colsurfb.2024.114338 (Original work published 2025)