Intracardiac allogeneic mesenchymal stem cell transplantation elicits neo-angiogenesis in a fully immunocompetent ischaemic swine model.
(2010) European Journal of Cardio-Thoracic Surgery — Vol. 38, n° 6, p. 781-787 (2010)
Files
PonceletEurJCardiothoracSurg38781.pdf
Restricted Access - Adobe PDF
- 514.7 KB
Details
- Authors
Zech, Francis 
Gianello, Pierre
- Abstract
- Objectives: Autologous mesenchymal stem cell transplantation has been shown to improve myocardial function in ischaemic cardiomyopathy. We studied one hypothetical mechanism, neo-angiogenesis, using allogeneic mesenchymal stem cell transplantation in an ischaemic swine model. Methods: Allogeneic mesenchymal stem cells were injected in the peri-infarct area (1x10(6)cellskg(-1)) 2 weeks after myocardial infarction. Myocardial infarction alone (n=3) served as a control group. In the myocardial infarction-mesenchymal stem cells group (n=6), tacrolimus was given from day 0 to day 12. Capillary density and inflammatory/rejection processes (anti-factor VIII and anti-CD3/CD68 monoclonal antibodies, respectively) were compared between groups. Results: In scarred myocardium, capillary density was similar between both ischaemic groups: 15.4 (+/-15.3) and 14.7 (+/-15.2) vessel/field in myocardial infarction-mesenchymal stem cells and myocardial infarction-alone groups (non-significant). In viable myocardium adjacent to the infarction, capillary density was significantly increased in the myocardial infarction-mesenchymal stem cells group than in the myocardial infarction-alone group (p=0.002). The number of infiltrating CD3+ cells was equivalent in both myocardial infarction-alone and myocardial infarction-mesenchymal stem cells groups (CD3+: 8.6% vs 9.3%, non-significant). However, CD68+ cell infiltration was more prominent after mesenchymal stem cell transplantation (4.7% vs 2% in myocardial infarction alone, p<0.01). Conclusions: Allogeneic mesenchymal stem cell transplantation enhances angiogenesis after myocardial infarction. This effect is limited to the viable myocardium. Using a concomitant 12-day course of tacrolimus, no mesenchymal stem cell-specific cellular immune response was demonstrated.
- Affiliations
Citations
Poncelet, A., Hiel, A.-L., Vercruysse, J., Hermans, D., Zech, F., & Gianello, P. (2010). Intracardiac allogeneic mesenchymal stem cell transplantation elicits neo-angiogenesis in a fully immunocompetent ischaemic swine model. European Journal of Cardio-Thoracic Surgery, 38(6), 781-787. https://doi.org/10.1016/j.ejcts.2010.03.035 (Original work published 2010)