Tyrosine phosphorylation of rabphilin during long-lasting long-term potentiation.
(2007) Neuroscience Letters — Vol. 414, n° 3, p. 257-262 (2007)
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- Long-term potentiation (LTP) is a persistent increase in the strength of synaptic transmission triggered by neuronal activity. Here, we submitted hippocampal slices to a perfusion of forskolin and IBMX, which induces a long-lasting LTP (>4 h) (L-LTP). We separated the proteins of the CA1 region by two-dimensional gel electrophoresis (2-DE). We then immunoblotted them using an anti-p-Tyr antibody. We found a protein whose tyrosine phosphorylation was unchanged 10 min after LTP induction but was dramatically increased after 1h, dropping back to its baseline after 4 h. This protein was identified as rabphilin using matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). We also demonstrated that genistein, an inhibitor of tyrosine phosphorylation, prevented the development of the late phase of electrically-induced L-LTP. Our results suggest that rabphilin, a protein present in presynaptic terminals, could play a role in the late phase of L-LTP.
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Capron, B., Wattiez, R., Sindic, C., Godaux, E., & Ris, L. (2007). Tyrosine phosphorylation of rabphilin during long-lasting long-term potentiation. Neuroscience Letters, 414(3), 257-262. https://doi.org/10.1016/j.neulet.2006.12.031 (Original work published 2007)