Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells
(2020) ACS Applied Bio Materials — Vol. 3, n° 3, p. 1520-1532 (2020)
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Durrieu, Marie-Christine 
- Abstract
- The development of a functional in vitro model for microcirculation is an unresolved challenge, with major impact for the creation and regeneration of organs in the tissue engineering. The absence of pre-vascularized engineered tissues limits enormously their efficacy and integration. Therefore, in this study, the in vitro formation of tubular-like structures with human umbilical vein endothelial cells (HUVECs) is investigated thanks to three-dimensional polycarbonate (PC) micro-channel (μCh) scaffolds), surface biofunctionalized with hyaluronic acid/chitosan (HA/CHI) layer-by-layer (LbL) films grafted with adhesive (RGD) and angiogenic (SVV and QK) peptides, alone and in combination. The novelty of this work lies in the formation of capillaries in the order of tens μm, developing spontaneous micro-vessels, without the complexity of micro-fluidic approaches, and in a short time-scale. Ellipsometry, confocal laser scanning microscopy and fluorospectrometry are used to characterize the biofunctionalized micro-channels. PC-μCh scaffolds functionalized with (HA/CHI)12.5 film (PC-LbL) and further grafted with RGD and QK peptides (PC-RGD+QK) or with RGD and SVV peptides (PC-RGD+SVV) are then tested for in vitro blood vessel formation. These assays evidence a rapid formation of tubular-like structures after 2h of incubation. Moreover, co-culture system involving HUVECs and human pericytes derived from placenta (hPCs-PL) stabilizes the tubes for longer time.
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Citations
Aor, B., Khan, I., Glinel, K., Jonas, A., Demoustier, S., & Durrieu, M.-C. (2020). Microchannel Molding Combined with Layer-by-Layer Approach for the Formation of Three-Dimensional Tube-like Structures by Endothelial Cells. ACS Applied Bio Materials, 3(3), 1520-1532. https://doi.org/10.1021/acsabm.9b01150 (Original work published 2020)