Production of [2-11C]thymidine for quantification of cellular proliferation with PET.

Vander Borght, Thierry;Labar, Daniel;Pauwels, Stanislas;Lambotte, Luc
(1991) International Journal of Radiation Applications and Instrumentation. Part A: Applied Radiation and Isotopes — Vol. 42, n° 1, p. 103-104 (1991)

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Authors
  • Vander Borght, ThierryUCLouvain
    Author
  • Labar, DanielUCLouvain
    Author
  • Pauwels, StanislasUCLouvain
    Author
  • Lambotte, LucUCLouvain
    Author
Abstract
A three-step synthesis of [2-11C]thymidine, using [11C]urea as precursor, is described. [2-11C]Thymine was obtained by cyclization of [11C]urea and diethyl beta-methylmalate in fuming sulfuric acid. After purification of the reaction mixture, [2-11C]thymine and 2'-deoxyribose-1-phosphate were incubated in the presence of thymidine phosphorylase to form [2-11C]thymidine. The whole synthesis procedure, including purification and pharmaceutical package, was achieved within 60 min with a decay corrected yield of 30-35%.
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Vander Borght, T., Labar, D., Pauwels, S., & Lambotte, L. (1991). Production of [2-11C]thymidine for quantification of cellular proliferation with PET. International Journal of Radiation Applications and Instrumentation. Part A: Applied Radiation and Isotopes, 42(1), 103-104. https://doi.org/10.1016/0883-2889(91)90131-J (Original work published 1991)