Role of rsbU and staphyloxanthin in phagocytosis and intracellular growth of staphylococcus aureus in human macrophages and endothelial cells

Olivier, Aurélie C.;Lemaire, Sandrine;Van Bambeke, Françoise;Tulkens, Paul M.;Oldfield, Eric
(2009) The Journal of Infectious Diseases — Vol. 200, n° 9, p. 1367-1370 (2009)

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Authors
  • Olivier, Aurélie C.UCLouvain
    Author
  • Lemaire, SandrineUCLouvain
    Author
  • Van Bambeke, Françoiseorcid-logoUCLouvain
    Author
  • Tulkens, Paul M.UCLouvain
    Author
  • Oldfield, Eric
    Author
Abstract
In Staphylococcus aureus, rsbU down-regulates agr and stimulates production of staphyloxanthin (STX), an antioxidant that may contribute to intracellular survival after phagocytosis. Using isogenic rsbU(-) and rsbU(+) strains, we show that rsbU causes increased internalization and intracellular growth in both THP-1 macrophages and human umbilical vein endothelial cells (more so for the latter) without change in subcellular localization and that inhibition of STX biosynthesis markedly reduces intracellular growth of the rsbU(+) strain (and of clinical isolates, including USA300; tested with macrophages only) without affecting internalization. Thus, rsbU is important for uptake and for STX biosynthesis and is critical for intracellular multiplication of S. aureus.
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Olivier, A. C., Lemaire, S., Van Bambeke, F., Tulkens, P. M., & Oldfield, E. (2009). Role of rsbU and staphyloxanthin in phagocytosis and intracellular growth of staphylococcus aureus in human macrophages and endothelial cells. The Journal of Infectious Diseases, 200(9), 1367-1370. https://doi.org/10.1086/606012 (Original work published 2009)