Gastric ATPase phosphorylation/dephosphorylation monitored by new FTIR-based BIA-ATR biosensors
(2010) Spectroscopy : an international journal — Vol. 24, n° 3-4, p. 257-260 (2010)
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- Abstract
- Biosensors are composite devices suitable for the investigation of receptor-ligand interactions. In this paper we present the specific application to a membrane embedded protein of a new sensor device, so-called BIA-ATR, based on Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy. It consists in a functionalised ATR germanium crystal whose surface has been covalently modified to adsorb a biomembrane. Detection of the ligand-receptor interaction is achieved using FTIR spectroscopy. We report the specific detection of the phosphorylation/dephosphorylation of the H+/K+ gastric ATPase. The H+, K+-ATPase is a particularly large protein entity. This glycosylated protein contains more than 1300 residues and is embedded in a lipid membrane. Yet we demonstrate that the BIA-ATR sensor is capable of monitoring the binding of a single phosphate on such a large protein entity. Furthermore, we also demonstrate the potential of the approach to monitor the kinetics of binding and dissociation of the ligand.
- Affiliations
ULB Lab Struct & Funct Biol Membranes, Ctr Struct Biol & Bioinformat, B-1050 Brussels
Citations
Goldsztein, A., Babar, S., Voue, M., De Coninck, J., Conti, J., Marchand-Brynaert, J., Devouge, S., Homblé, F., & Goormaghtigh, E. (2010). Gastric ATPase phosphorylation/dephosphorylation monitored by new FTIR-based BIA-ATR biosensors. Spectroscopy : an international journal, 24(3-4), 257-260. https://doi.org/10.3233/SPE-2010-0402 (Original work published 2010)