A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium.
(1987) Microbial Pathogenesis — Vol. 3, n° 2, p. 109-116 (1987)
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- Abstract
- A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
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Michiels, T., Popoff, M. Y., Durviaux, S., Coynault, C., & Cornelis, G. R. (1987). A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium. Microbial Pathogenesis, 3(2), 109-116. https://doi.org/10.1016/0882-4010(87)90069-6 (Original work published 1987)