Immunoassay of theophylline by latex particle counting
(1990) Journal of Immunoassay — Vol. 11, n° 1, p. 77-88 (1990)
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- Abstract
- We set up an immunoassay by particle counting for theophylline. Theophylline concentration is assayed by its capacity to inhibit the agglutination of theophylline coated latex particles by a specific monoclonal antibody, the agglutination being enhanced by a rabbit anti-mouse IgG antiserum. The dose range is 2-64 mg/L. The cross-reactions observed with caffeine (0.3%), theobromine (0.2%), 3-methylxanthine (0.7%) and 8-chlorotheophylline (2%) are very good when compared with other published methods. Within and between-run precisions measured at low, medium and high level of the calibration curve show coefficients of variation ranging from 3.9% to 9.5%. Our assay was correlated with the Fluorescence Polarization Immunoassay (FPIA) and a correlation coefficient of 0.96 was determined for 89 samples.
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Poncelet, S., Limet, J., Noel, J., Kayeart, M., Galanti, L., & Collet-Cassart, D. (1990). Immunoassay of theophylline by latex particle counting. Journal of Immunoassay, 11(1), 77-88. https://hdl.handle.net/2078.5/54984 (Original work published 1990)