Crystal growth of C45S mutant of Arenicola marina peroxiredoxin 6 in microgravity environment. : An unexpected benefit of convection on crystal quality.

Smeets, Aude;Knoops, Bernard;Declercq, Jean-Paul
(2008) JASM A: Journal of the Japan Society of Microgravity Application — Vol. 25, n° 3, p. 603-606 (2008)

Files

SmeetsalPrInt.pdf
  • Restricted Access
  • Adobe PDF
  • 455.95 KB
Request a copy

Details

Authors
  • Smeets, AudeUCLouvain
    Author
  • Knoops, BernardUCLouvain
    Author
  • Declercq, Jean-PaulUCLouvain
    Author
Abstract
Crystallization is the first step in the structure determination by X-ray. Crystallization under microgravity conditions (without convection) could have consequences for the crystal growth and for the quality of crystals. We report here, the results of the crystallization ofArenicola marina peroxiredoxin 6 in microgravity environment. This protein was chosen to participate to the F0T0N-M3 mission. There are two crystal forms of Arenicola marina peroxiredoxin 6, under the same crystallization conditions and the results of the experiment seem to show that crystals obtained without convection, present a mixture of different zones belonging to the two crystal forms. Surprisingly, the results suggest that convection is necessary to select one single crystal form in one crystal.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Smeets, A., Knoops, B., & Declercq, J.-P. (2008). Crystal growth of C45S mutant of Arenicola marina peroxiredoxin 6 in microgravity environment. : An unexpected benefit of convection on crystal quality. JASM A: Journal of the Japan Society of Microgravity Application, 25(3), 603-606. https://hdl.handle.net/2078.5/71118 (Original work published 2008)