New BNYVV P25 variants in Belgium
(2015) Communications in Agricultural and Applied Biological Sciences — Vol. 80, n° 3, p. 407-409 (2015)
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- Authors
Decroës, Alain 
- Abstract
- Rhizomania is a widespread viral plant disease of major importance in sugar beet crop and breeding. It is caused by the Beet necrotic yellow vein virus (BNYVV), a Benyvirus transmitted by the soil inhabiting plasmodiophorid Polymyxa betae. This vector also transmits other sugar beet virus such as Beet virus Q (BVQ) and Beet soil-borne virus (BSBV). Despite identification of resistance genes, BNYVV remains a major constraint because of resistance breaking events as well as its ability to survive for long periods in soils in resting spores of P. betae. During the 2014 growing season, severe rhizomania symptoms were detected on Rz1 resistant genotypes in ten fields in Belgium suggesting resistance-breaking events. Plants from these fields were sampled and total RNA was extracted from root hairs. The presence of BNYVV, BSBV, BVQ and P. betae was assessed by multiplex RT-PCR. Samples were then tested for the presence of RNA 5 and RNA 3 by RT-PCR respectively targeting P26 and P25 genes. PCR products for P25 were then purified and sequenced. The results confirmed the presence of P. betae, BSBV and BVQ in all samples. BNYVV was detected in nine fields. Sequencing of P25 partial cDNA sequences revealed the presence of BNYVV types A and B. Two isolates possessed amino acids motifs AYPR in the so-called tetrad region aa67-70. This motif was previously associated with resistance breaking events. The Belgian situation will be discussed in the light of the current situation in neighbouring countries.
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Citations
Decroës, A., Clausse, M., Galein, Y., Legrève, A., wauters, A., & Bragard, C. (2015). New BNYVV P25 variants in Belgium. Communications in Agricultural and Applied Biological Sciences, 80(3), 407-409. https://hdl.handle.net/2078.5/255790 (Original work published 2015)