Development of a large set of diagnostic SNP markers using ddRAD-seq to study hybridization in Rhinanthus major and R. minor (Orobanchaceae)

Mirzaei, Khaled;Wesselingh, Renate
(2021) Conservation Genetics Resources — Vol. 13, p. 31-33 (2021)

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Rhinanthus major and R. minor are two annual plant species that are known to hybridize in nature and in which unilateral introgression is likely to occur. Here we used double-digest restriction site-associated DNA sequencing technology (ddRAD-seq) to detect 16,932 genome-wide SNPs in R. major and R. minor individuals from 9 populations. After screening these SNP markers across both species, we selected 1106 putative loci that contain diagnostic, species-specific SNPs, which can be used for assessing and monitoring hybridization and introgression between R. major and R. minor.
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Mirzaei, K., & Wesselingh, R. (2021). Development of a large set of diagnostic SNP markers using ddRAD-seq to study hybridization in Rhinanthus major and R. minor (Orobanchaceae). Conservation Genetics Resources, 13, 31-33. https://doi.org/10.1007/s12686-020-01181-5 (Original work published 2021)