Biosynthesis of selemium nanobars by Shewallena oneidensis MR-1
(2015) Weishengwu Xuebao — Vol. 55, n° 8, p. 1074-1078 (2015)
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- Abstract
- OBJECTIVE: We used Shewallena oneidensis MR-1 to produce selemium (Se) nanobars and studied the influence of Se(IV) concentrations and incubation time on nanobars production.; METHODS: We incubated Shewallena oneidensis MR-1 under anaerobic condition with Luria-Bertani (LB) liquid medium containing 0.1, 1.0, 10.0 or 100.0 mmol/L Se (IV) in Na2SeO3, to determine the optimal Se (IV) concentration for bacterial growth. Then, we incubated Shewallena oneidensis MR-1 with the optimal Se (IV) concentration and collected deposits 24 and 72 h after anearobic incubation. We used scanning electron microscopy, energy-dispersive X-ray and X-ray diffraction to analyse the deposits.; RESULTS: The cross sectional diameter and length of deposits that were produced by Shewallena oneidensis MR-1 after 24 h incubation with 1 mmol/L Se(IV) was around 80 nm and 2-3 μm, respectively. However, the deposits after 72 h incubation exceeded the size limit of nano material. Furthermore, the energy-dispersive X-ray and the X-ray diffraction spectroscopy confirmed that the deposits were elemental Se.; CONCLUSION: This study provides a viable method for the biosynthesis of Se nanoban Shewallena oneidensis MR-1 can produce a large number of Se nanobars at exponential phase under 0.1 mmol/L Se (IV).
- Affiliations
College of Environmental Science and Engineering Hunan University
Citations
Deng, H., Zheng, Z., & Zhao, F. (2015). Biosynthesis of selemium nanobars by Shewallena oneidensis MR-1. Weishengwu Xuebao, 55(8), 1074-1078. https://hdl.handle.net/2078.5/25674 (Original work published 2015)