In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots
(1996) International Journal of Orthopaedic and Trauma Nursing — Vol. 100, n° 10, p. 1237-1242 (1996)
Files
1-s20-S0953756296801869-main.pdf
Restricted Access - Adobe PDF
- 2.47 MB
Details
- Authors
- Abstract
- he rate of in vitro spore formation of the arbuscular mycorrhizal fungus Glomus versiforme was followed in Petri dishes, using mycorrhizal root-segment inoculum associated with Ri T-DNA transformed carrot roots. Three phases of sporulation were observed: a lag phase, a period of intensive spore production and a plateau phase. An average of 9500 spores per Petri dish was produced after 5 months of dual culture. The root-organ culture system supported extensive root colonization, with many arbuscules and vesicles being formed. The fungus, both within root-segments and as spores produced, was viable and able to complete its life cycle in vitro. The mycorrhizal root-segments, however, exhibited higher inoculum potential due to the numerous vesicles and extensive intraradical mycelium.
- Affiliations
Citations
Declerck, S., Strullu, D. G., & Plenchette, C. (1996). In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots. International Journal of Orthopaedic and Trauma Nursing, 100(10), 1237-1242. https://doi.org/10.1016/S0953-7562(96)80186-9 (Original work published 1996)