Secretion of sucrase by Leishmania donovani
(1994) The Journal of Eukaryotic Microbiology — Vol. 41, n° 3, p. 228-231 (1994)
Files
No attached file found for this publication.
Details
- Authors
Opperdoes, Frederik 
- Abstract
- Leishmania donovani promastigotes were collected, washed, resuspended in buffer, and assayed for sucrase activity. No activity was observed in the intact washed cells, but activity was measurable when the cells were permeabilized with Triton X-100. Intracellular sucrase activity was highest in promastigotes grown at pH 7.4, somewhat lower in promastigotes grown at pH 5.5, and significantly lower in "amastigotes" grown at pH 5.5. No trehalase, lactase, or maltase activities were observed. Assay of the medium in which the cells had grown showed that most the sucrase activity was extracellular, i.e. was secreted into the medium during growth.
- Affiliations
Citations
Blum, J. J., & Opperdoes, F. (1994). Secretion of sucrase by Leishmania donovani. The Journal of Eukaryotic Microbiology, 41(3), 228-231. https://doi.org/10.1111/j.1550-7408.1994.tb01502.x (Original work published 1994)