Synthesis, Purification and Kinetic-properties of Fluorescein-labeled Penicillins
(1994) Biochemical Journal — Vol. 300, p. 141-145 (1994)
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- Abstract
- The synthesis and properties of six fluorescein-labelled penicillins are reported. The two isomers of fluoresceyl-glycyl-6-amino-penicillanic acid are probably the best compounds to use for detection of all the penicillin-binding proteins (PBPs) present in a bacterial membrane preparation. However, the derivatives of ampicillin were much more efficient against Enterobacter aerogenes PBP3. The two isomers obtained when a commercial mixture of the two isomers of carboxyfluorescein was used most often exhibited similar properties, but the Streptomyces R61 extracellular DD-peptidase was only efficiently acylated by the 5'-carboxyfluorescein derivative of glycyl-6-aminopenicillanic acid.
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Lakaye, B., Damblon, C., Jamin, M., Galleni, M., Lepage, S., Joris, B., Marchand-Brynaert, J., Frydrych, C., & Frère, J.-M. (1994). Synthesis, Purification and Kinetic-properties of Fluorescein-labeled Penicillins. Biochemical Journal, 300, 141-145. https://hdl.handle.net/2078.5/71840 (Original work published 1994)