Development of antral follicles after xenografting of isolated small human preantral follicles.
(2008) Reproductive BioMedicine Online — Vol. 16, n° 5, p. 705-711 (2008)
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- Small human pre-antral follicles can be enzymatically isolated from the surrounding stroma, and are able to survive after 7 days of xenografting. The aim of the present study was to assess the developmental capacity of enzymatically isolated human follicles after long-term xenografting to severe combined immunodeficient (SCID) mice. Ovarian biopsies were obtained from three women 26-29 years of age. Human ovarian tissue was enzymatically dissociated using collagenase or a purified collagenase blend to obtain isolated follicles that were xenografted to SCID mice for 5 months. Recombinant FSH was given to the mice for the last 2 weeks. Five months after xenografting, follicular morphology was assessed by histology, and follicular proliferation by Ki-67 immunohistochemistry. Four grafts containing a total of 84 follicles were recovered. This follicular population was composed of 11 primordial follicles, 38 primary follicles, 31 secondary follicles and four antral follicles. Ki-67 was found to stain granulosa cells in antral follicles intensively. The results demonstrate, for the first time, that isolated human follicles are able to survive after long-term xenografting, and can develop into antral follicles after FSH stimulation.
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Dolmans, M.-M., Yuan, W. Y., Camboni, A., Torre, A., Van Langendonckt, A., Martinez-Madrid, B., & Donnez, J. (2008). Development of antral follicles after xenografting of isolated small human preantral follicles. Reproductive BioMedicine Online, 16(5), 705-711. https://doi.org/10.1016/S1472-6483(10)60485-3 (Original work published 2008)