Impact of efflux mechanisms on susceptibility and resistance to beta-lactam antibiotics in Pseudomonas aeruginosa : beyond the usual concepts
(2016)
Files
ChalhoubHussein-PhDthesis.pdf
Open Access - Adobe PDF
- 10.54 MB
Details
- Authors
- Supervisors
- Van Bambeke, Françoise
- Abstract
- The objective of the thesis was to evaluate the active efflux as a mechanism of resistance to β-lactams in Pseudomonas aeruginosa isolated from European patients with cystic fibrosis. Our findings emphasize the increased prevalence of multidrug-resistant isolates. They also demonstrate that active efflux plays a major role in β-lactam resistance. In particular, active efflux confers high-level resistance to meropenem and avibactam (new β-lactamase inhibitor), when combined with alterations in OprD porins. Furthermore, natural mutations in the genes encoding MexAB-OprM efflux pump restore the activity of temocillin (considered as inactive against P. aeruginosa) against a non-negligible proportion of isolates. This work re-examines the concept that efflux confers only low-level resistance. In addition, it allows to better position the interest of antibiotics used in the context of cystic fibrosis.
- Affiliations
Citations
Chalhoub, H. (2016). Impact of efflux mechanisms on susceptibility and resistance to beta-lactam antibiotics in Pseudomonas aeruginosa : beyond the usual concepts. https://hdl.handle.net/2078.5/38555