Determination of Leucovorin and 5-fluorouracil in Plasma By High-performance Liquid-chromatography
(1993) Journal of Chromatography. Biomedical Applications — Vol. 612, n° 1, p. 77-85 (1993)
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- Abstract
- A method was developed for the determination of (6R)- and (6S)-leucovorin and 5-fluorouracil in plasma. As leucovorin diastereoisomers cannot be separated on a classical reversed-phase column, it was necessary to use a chiral stationary phase. The method presented is based on the same principle as the method described by Wainer and Stiffin [J. Chromatogr., 424 (1988) 158], i.e., coupling of a bovine serum albumin phase to an achiral stationary phase. Before the chromatography, the drug was isolated from the plasma matrix by solid-phase extraction. For 5-fluorouracil, chromatography was performed on a classical RP-18 column after extraction from the plasma by liquid-liquid extraction. Both methods were validated and applied to the analysis of patients' samples.
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Citations
Vandenbosch, C., Vanbelle, S., De Smet, M., Taton, G., Bruynseels, V., Vandenhoven, G., & Massart, DL. (1993). Determination of Leucovorin and 5-fluorouracil in Plasma By High-performance Liquid-chromatography. Journal of Chromatography. Biomedical Applications, 612(1), 77-85. https://doi.org/10.1016/0378-4347(93)80370-J (Original work published 1993)