A principled approach and standardised software for mass spectrometry-based single-cell proteomics data analysis
(2024)
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- Recent advances in sample preparation and mass spectrometry (MS) have enabled the emergence of quantitative MS-based single-cell proteomics (SCP). However, the analysis of SCP data is challenging and must address numerous problems that are inherent to both MS-based proteomics technologies and single-cell experiments. Through the development of standardised software and data, this work establishes the foundation for SCP data analysis. Our efforts have led to a comprehensive identification and understanding of the obstacles hindering the accurate extraction of biologically meaningful information from these complex data. Consequently, we have developed a computational approach explicitly designed to address these challenges, facilitating a seamless analysis of SCP data. This work reshapes the analysis of SCP data by moving efforts from dealing with the technical aspects of data analysis to focusing on answering biologically relevant questions.
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Vanderaa, C. (2024). A principled approach and standardised software for mass spectrometry-based single-cell proteomics data analysis. https://hdl.handle.net/2078.5/29780