Conserved TCR usage by HLA-Cw*1601-restricted T cell clones recognizing melanoma antigens
(1996) International Immunology — Vol. 8, n° 9, p. 1463-1466 (1996)
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- Abstract
- In this study we determined TCR alpha and beta chain nucleotide sequences of HLA-Cw*1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, >1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the alpha chain CDR3 for two residues and in the beta chain CDR3 for a single nucleotide which, however, did not change translation, These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.
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Citations
Farina, C., van der Bruggen, P., Boël, P., Parmiani, G., & Sensi, M. (1996). Conserved TCR usage by HLA-Cw*1601-restricted T cell clones recognizing melanoma antigens. International Immunology, 8(9), 1463-1466. https://doi.org/10.1093/intimm/8.9.1463 (Original work published 1996)