Intraspecies variation in Staphylococcus epidermidis adhesion onto coated and uncoated hydrophobic materials
(2013) Eurobiofilms 2013 — Location: Gand (9.September.2013)
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- Abstract
- Since early 1980's, the attention paid to Staphylococcus epidermidis as a nosocomial pathogen has steadily increased. Biofilms of this species occur after initial adhesion of bacteria to medical implant and are highly resilient in the human body, even when challenged with antibiotics. Understanding the processes that govern S. epidermidis adhesion is critical to prevent infections on biomaterials. In this study, the adhesion of S. epidermidis strains isolated from blood cultures, healthy individuals or inert surfaces was investigated in multiwell plates for 22 strains (Polystyrene, PS) and in flow cells for 4 strains (Polydimethylsiloxane, PDMS). Stationary phase cultures suspended in Phosphate Buffer Saline (PBS) were set in contact with PS, either uncoated or coated with Trypticase Soy Broth (TSB), Albumin, Fibrinogen or diluted bovine serum (BS) and with PDMS, uncoated or coated with TSB or BS. The results in both static and dynamic conditions show a clear tendency of uncoated hydrophobic materials to promote adhesion whereas surfaces coated with BS significantly reduced adhesion for all tested strains by up to 100 fold. However, on a TSB-coated surface, some strains adhered in the same fashion as on an uncoated hydrophobic material while other strains showed a significantly decreased adhesion. These intra-species discrepancies could be statistically explained by coating-strain interactions. In other words, two S. epidermidis strains may interact differently with surfaces of changing physicochemical properties. Interestingly also, even though some strains possess the genes that encode adhesion recognizing fibrinogen, surface coated with this protein was the most inhibiting for S. epidermidis adhesion. This could indicate a low level of expression of adhesive factors in vitro. In conclusion, these results tend to show that S. epidermidis adhesion ability to adhere on hydrophobic surfaces is globally reduced with the latter are coated with proteins, corroborating previous evidence in the literature. This study also highlighted the dependency of S. epidermidis adhesion on the strain tested, a fact of great importance in the development of anti-adhesive biomaterials.
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Citations
Vanzieleghem, T., Nannan, C., Herman, P., Dupont, C., Dufrêne, Y., Mahillon, J., & et al. (2013). Intraspecies variation in Staphylococcus epidermidis adhesion onto coated and uncoated hydrophobic materials. Eurobiofilms 2013, Gand. https://hdl.handle.net/2078.5/201474