Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes
(2002) Journal of Fish Biology — Vol. 61, n° 1, p. 71-84 (2002)
Files
No attached file found for this publication.
Details
- Authors
- Abstract
- The haemolysis of sea bass Dicentrarchus labrax red blood cells (RBC) was initiated by tert-butyl-hydroperoxide (t-BHP). The onset of the haemolytic process, as accelerated by increasing t-BHP concentration, This process as preceded by a drop in the RBC glutathione content followed by the production of lipid peroxidation products. Also t-BHP induced DNA fragmentation in RBC nuclei as measured by COMET assay. The addition of the antioxidant Trolox C-R dose-dependent delayed the onset of both lipid peroxidation and haemolysis, and protected GSH stores against t-BHP-induced depletion. DNA fragmentation was also prevented by Trolox C-R. These results indicate that L-BHP induces haemolysis in sea bass RBC through the induction of oxidative stress Such a simple model Could prove useful for both fundamental and applied studies on marine fish antioxidant mechanisms. (C) 2002 The Fisheries Society of the British Isles, Published by Elsevier Science Ltd. All rights reserved.
- Affiliations
Citations
Janssens, B., Le Gall, R., & Rees, J.-F. (2002). Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes. Journal of Fish Biology, 61(1), 71-84. https://doi.org/10.1006/jfbi.2002.2008 (Original work published 2002)