Arbuscular Mycorrhizal Fungi Enhanced Drought Resistance of Populus cathayana by Regulating the 14-3-3 Family Protein Genes
(2022) Microbiology Spectrum — Vol. 10, n° 3, p. e02456-21 (2022)
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- Abstract
- Plants can improve their resistance to a variety of stresses by forming mutualistic relationships with arbuscular mycorrhizal fungi (AMF). The 14-3-3 protein is a major regulator of the plant stress response. However, the regulation mechanism of 14-3-3 family protein genes (14-3-3s) of mycorrhizal plants coping with stress during AMF symbiosis remains unclear. Here, we analyzed the physiological changes and 14-3-3 expression profiles of Populus cathayana inoculated with AMF under different water conditions. The results showed that good colonization and symbiotic relationships with plants were formed under all water conditions (63.00% to 83.67%). Photosynthesis, peroxidase (POD) activity, and Mg and Ca content were significantly affected by drought and AMF. In addition, thirteen 14-3-3 protein genes (PcGRF1-PcGRF13) were identified by quantitative real-time PCR (qRT-PCR), of which the expression levels of PcGRF10 and PcGRF11 induced by AMF were significantly positively correlated with superoxide dismutase (SOD), POD, and sugar content, indicating that the 14-3-3s of mycorrhizal symbiotic plants may respond to drought through antioxidant and osmotic regulation. This is the first study on 14-3-3s in the symbiosis system of forest arbor plants and AMF, and it may help to further study the effects of 14-3-3s during AMF symbiosis on stresses and provide new ideas for improving mycorrhizal seedling cultivation under stress.
- Affiliations
Forestry Institute Northwest Agriculture & Forestry University
Citations
Han, Y., Lou, X., Zhang, W., Xu, T., Tang, M., & Wang, Y. (2022). Arbuscular Mycorrhizal Fungi Enhanced Drought Resistance of Populus cathayana by Regulating the 14-3-3 Family Protein Genes. Microbiology Spectrum, 10(3), e02456-21. https://doi.org/10.1128/spectrum.02456-21 (Original work published 2022)