Adenosine protects ischemic and reperfused myocardium by receptor-mediated mechanisms.

Janier, Marc;Vanoverschelde, Jean-Louis;Bergmann, Steve R.
(1993) American journal of physiology — Vol. 264, n° 1 Pt 2, p. H163-70 (1993)

Files

AmJPhysiol1993264H163-H270.pdf
  • Restricted Access
  • Adobe PDF
  • 1.97 MB
Request a copy

Details

Authors
  • Janier, MarcWashington University
    Author
  • Vanoverschelde, Jean-LouisUCLouvain
    Author
  • Bergmann, Steve R.Washington University
    Author
Abstract
To evaluate the role of adenosine receptors in the mediation of adenosine-induced protection of the heart during ischemia and reperfusion, isolated rabbit hearts were perfused at constant flow with 1 microM adenosine started before low-flow ischemia followed by reperfusion. Adenosine delayed the time of onset of ischemic contracture [to 28 +/- 19 (SD) min compared with 10 +/- 10 min in control hearts] and decreased the amplitude of ischemic contracture (29 +/- 16 vs. 48 +/- 14 mmHg; P < 0.05 for each compared with controls). This protection was accompanied by an increase in tissue ATP content (1.72 +/- 0.78 vs. 0.96 +/- 0.23 mumol/g; P < 0.05) and stimulation of anaerobic glycolysis (lactate production of 0.78 +/- 0.28 mumol.g-1 x min-1 compared with 0.53 +/- 0.23 mumol.g-1 x min-1; P < 0.05). Functional recovery during reperfusion was enhanced by adenosine (developed pressure 88 +/- 16% compared with 57 +/- 23% of baseline; P < 0.05), and tissue necrosis, assessed by creatine kinase release, was decreased. The potent, nonselective adenosine receptor blocker 8-phenyltheophylline (10 microM) blocked all of the salutary effects of adenosine. Adenosine given only at reperfusion modestly attenuated reperfusion-induced contracture. The results suggest that exogenous adenosine attenuates ischemic injury by receptor-mediated stimulation of anaerobic glycolysis. During reperfusion its protective action is related to vasodilation.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Janier, M., Vanoverschelde, J.-L., & Bergmann, S. R. (1993). Adenosine protects ischemic and reperfused myocardium by receptor-mediated mechanisms. American journal of physiology, 264(1 Pt 2), H163-70. https://hdl.handle.net/2078.5/209395 (Original work published 1993)