Identification and characterization of the root cap cuticle of Arabidopsis thaliana
(2017) Plant Apoplastic Diffusion Barriers Conference — Location: Oeiras, Portugal (13.September.2017)
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- Abstract
- A cuticle is present on the surface of epidermal cells of aerial parts of the plant. Genes encoding key steps of the biosynthetic pathway of the cuticular polyester cutin have been characterized in Arabidopsis. Intriguingly, several genes that are involved in cutin formation are not only expressed in epidermal cells of the shoot, but also at the root cap of the primary root and/or of the lateral root, e.g. DCR, ABCG11, BDG. Transmission electron microscopy performed on 5-day old Arabidopsis roots revealed that the columella and the lateral root cap cells of the primary root as well as the emerging lateral root have an electron-dense layer on the surface of the outer most cell wall that has great similarity with the Arabidopsis leaf cuticle. The so-called Root Cap Cuticle (RCC) is continuously present from day1 to day5 after germination suggesting that it is present on several layers of mature root cap cells. Several Arabidopsis mutants having a knockout in cutin biosynthesis genes that are expressed at the root cap have an altered RCC ultrastructure suggesting that the electron-dense layer may be indeed of lipidic nature. The expression pattern of glycerol-3-phosphate acyltransferase (GPAT) family members indicated that GPAT2 and GPAT3 may be involved in RCC formation. Indeed, the gpat2 mutant has a modified RCC ultrastructure assigning a role to this particular GPAT-family branch. Studies to reveal the role of the RCC in root physiology and its chemically nature will be presented.
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Berhin, A., & et al. (2017). Identification and characterization of the root cap cuticle of Arabidopsis thaliana. Plant Apoplastic Diffusion Barriers Conference, Oeiras, Portugal. https://hdl.handle.net/2078.5/246989