Positive Selection of Recombinant Plasmids Based On the Ecok Restriction Activity of Escherichia-coli K-12
(1994) Gene — Vol. 150, n° 1, p. 197-198 (1994)
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- Abstract
- We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12. to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.
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Citations
De Backer, O., Chomez, P., & De Plaen, E. (1994). Positive Selection of Recombinant Plasmids Based On the Ecok Restriction Activity of Escherichia-coli K-12. Gene, 150(1), 197-198. https://doi.org/10.1016/0378-1119(94)90885-0 (Original work published 1994)