Atomic force microscopy of supported lipid bilayers

Mingeot-Leclercq, Marie-Paule;Deleu, Magali;Brasseur, Robert;Dufrêne, Yves
(2008) Nature Protocols (Online) : recipes for researchers — Vol. 3, n° 10, p. 1654-1659 (2008)

Files

2008-Mingeot-Dufrene-NatureProtocols.pdf
  • Restricted Access
  • Adobe PDF
  • 718.23 KB
Request a copy

Details

Authors
  • Mingeot-Leclercq, Marie-PauleUCLouvain
    Author
  • Deleu, Magali
    Author
  • Brasseur, Robert
    Author
  • Dufrêne, YvesUCLouvain
    Author
Abstract
Supported lipid bilayers (SLBs) are widely used in biophysical research to investigate the properties of biological membranes and offer exciting prospects in nanobiotechnology. Atomic force microscopy (AFM) has become a well-established technique for imaging SLBs at nanometer resolution. A unique feature of AFM is its ability to monitor dynamic processes, such as the interaction of bilayers with proteins and drugs. Here, we present protocols for preparing dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers supported on mica using small unilamellar vesicles and for imaging their nanoscale interaction with the antibiotic azithromycin using AFM. The entire protocol can be completed in 10 h.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Mingeot-Leclercq, M.-P., Deleu, M., Brasseur, R., & Dufrêne, Y. (2008). Atomic force microscopy of supported lipid bilayers. Nature Protocols (Online) : recipes for researchers, 3(10), 1654-1659. https://doi.org/10.1038/nprot.2008.149 (Original work published 2008)