After a stroke, around 80% of individuals suffer from upper limb motor impairments leading to an activity limitation and a reduced quality of life. To optimise rehabilitation and better predict recovery, it is recommended that patients be evaluated regularly. In this PhD thesis, different virtual applications allowing for the assessment of post-stroke manual dexterity, upper limb capacity and forearm kinematics have been validated. Results of these studies brought to light the potential of immersive virtual reality to deliver autonomous and objective assessments. Several meta-analyses were also conducted to assess the effect of virtual reality, robot-assisted therapy, serious games, self- and tele-rehabilitation programs in the treatment of individuals with stroke. Results of these works now open new perspectives for the use of technological means in the assessment and rehabilitation of individuals with stroke.