C/EBP binds over the TATA box and can activate the M promoter of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Vandoolaeghe, Pascale;Rousseau, Guy
(1997) Biochemical and Biophysical Research Communications — Vol. 232, n° 1, p. 247-250 (1997)

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Authors
  • Vandoolaeghe, Pascale
    Author
  • Rousseau, GuyUCLouvain
    Author
Abstract
The gene A coding for 6-phosphofructo-2-kinase gives rise to three mRNA that originate from distinct promoters called F (fetal), M (muscle) and L (liver). The regulation of the M promoter is ill-understood and its TATA box region binds an unidentified factor. The aim of this work was to identify this factor and to investigate its activity. In vitro protein-DNA binding assays and transfection experiments showed that this factor is C/EBP and that C/EBP can stimulate the M promoter despite its potential interference with TFIID binding. The effect of C/EBP was abolished by a dominant negative variant of C/EBP. These data and other work lead to the conclusion that C/EBP may participate to the regulation of promoter switching in 6-phosphofructo-2-kinase gene A.
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Vandoolaeghe, P., & Rousseau, G. (1997). C/EBP binds over the TATA box and can activate the M promoter of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Biochemical and Biophysical Research Communications, 232(1), 247-250. https://doi.org/10.1006/bbrc.1997.6266 (Original work published 1997)