The influence of four agar-type polysaccharides, used as solidifying agents for culture media and differing in their sulfate content (0.14 to 10.95 % w/w), was tested on the budding and the elongation of five clones of Thuja plicata. Budding is reduced on the most sulfated polysaccharide and the differences observed between clones in elongation are as important as those observed between agar types.
Pochet, B., Scoman, V., Mestdagh, MM., Moreau, B., & André, P. (1991). Influence of Agar-gel Properties On the Invitro Micropropagation of Different Clones of Thuja-plicata. Plant Cell Reports, 10(8), 406-409. https://doi.org/10.1007/BF00232612 (Original work published 1991)