Cloning of the cDNA from normal brain and brain of patients with Alzheimer's disease in the expression vector lambda GT 11.
(1988) Progress in Neuro-Psychopharmacology & Biological Psychiatry — Vol. 12, n° 5, p. 813-820 (1988)
Files
No attached file found for this publication.
Details
- Authors
- Abstract
- 1. RNA was purified from postmortem human brains, and the poly A+ RNA was isolated by oligo dT cellulose. 2. Double stranded cDNA was synthesized using reverse transcriptase, RNAse H and DNA polymerase. 3. cDNA was cloned in the lambda GT 11 expression vector, and libraries containing between 1 and 2 millions clones were obtained. 92 to 98% of the plaques contained a recombinant phage. 4. Such libraries will allow the molecular characterization of cDNA and corresponding proteins which play a key role in brain functions and in particular which could be involved in the etiology of Alzheimer's dementia.
- Affiliations
Citations
Octave, J.-N., de Sauvage, F., Macq, A. F., & Maloteaux, J.-M. (1988). Cloning of the cDNA from normal brain and brain of patients with Alzheimer’s disease in the expression vector lambda GT 11. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 12(5), 813-820. https://doi.org/10.1016/0278-5846(88)90026-7 (Original work published 1988)