Preparation and characterization of protein-loaded poly(epsilon-caprolactone) microparticles for oral vaccine delivery.
(1999) International Journal of Pharmaceutics — Vol. 184, n° 1, p. 73-84 (1999)
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- Abstract
- This paper describes the conditions of preparation of poly(epsilon-caprolactone) (PCL) microparticles with a mean size between 5 and 10 microm, obtained by a double emulsion-solvent evaporation technique, suitable for oral vaccine delivery. Bovine serum albumin (BSA) was used as water-soluble model antigen for encapsulation. Different parameters influencing the microparticle size, the BSA loading and entrapment efficiency were investigated. Spherical, smooth and homogeneously distributed microparticles were produced with a BSA loading and entrapment efficiency reaching, respectively, 5% (w/w) and 30%. Polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF) analyses of BSA released from these particles confirmed that the entrapped protein seemed to remain unaltered by the protein encapsulation process. Copyright
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Benoit, M. A., Baras, B., & Gillard, J. (1999). Preparation and characterization of protein-loaded poly(epsilon-caprolactone) microparticles for oral vaccine delivery. International Journal of Pharmaceutics, 184(1), 73-84. https://doi.org/10.1016/S0378-5173(99)00109-X (Original work published 1999)