Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase.

Goffin, Philippe;Lorquet, Frédérique;Kleerebezem, Michiel;Hols, Pascal
(2004) Journal of bacteriology — Vol. 186, n° 19, p. 6661-6666 (2004)

Files

15375150.pdf
  • Open Access
  • Adobe PDF
  • 197.21 KB
Download

Details

Authors
  • Goffin, Philippe
    Author
  • Lorquet, Frédérique
    Author
  • Kleerebezem, Michiel
    Author
  • Hols, Pascalorcid-logoUCLouvain
    Author
Abstract
NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate conversion was not impaired in these strains, while it was completely blocked in mutants deficient in NAD-dependent lactate dehydrogenase activities, encoded by the ldhD and ldhL genes. We conclude that NAD-dependent but not NAD-independent lactate dehydrogenases are involved in this process.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Goffin, P., Lorquet, F., Kleerebezem, M., & Hols, P. (2004). Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase. Journal of bacteriology, 186(19), 6661-6666. https://doi.org/10.1128/JB.186.19.6661-6666.2004 (Original work published 2004)