Particle-counting immunoassay of human somatotropin.
(1984) Clinical Chemistry : international journal of molecular diagnostics and laboratory medicine — Vol. 30, n° 5, p. 672-676 (1984)
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- Abstract
- Human somatotropin was assayed by a novel automated nonradioisotopic technique, "particle-counting immunoassay," that requires a 45-min incubation and only 60 microL of 10-fold diluted sample. The principle of the assay is agglutination of antibody-coated latex particle by somatotropin, the reaction being measured by the (instrumented) counting of residual non-agglutinated particles. The dynamic range in serum extends from 0.2 to 40 micrograms/L. The between-assay CV was 12% for a concentration of 3.3 micrograms/L and 8.5% for 31.9 micrograms/L. The coefficient of correlation (r) with radioimmunoassay was 0.97. Curves for various dilutions of the macromolecular and monomeric forms of the hormone were not parallel.
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Citations
Castracane, C. E., Cambiaso, C., Retegui, L. A., Gilbert, I., Ketelslegers, J.-M., & Masson, P. (1984). Particle-counting immunoassay of human somatotropin. Clinical Chemistry : international journal of molecular diagnostics and laboratory medicine, 30(5), 672-676. https://hdl.handle.net/2078.5/133298 (Original work published 1984)