Contribution of plasma membrane lipid domains to red blood cell (re)shaping
(2017)
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- Abstract
- Cells exhibit a wide diversity of shapes and are subjected to constant remodeling, allowing for their specific functions. A typical example is the erythrocyte, which possesses a specific biconcave shape and remarkable deformability allowing squeezing through narrow splenic pores. In senescence and spherocytosis, a genetic membrane deformability disorder, erythrocyte fails to deform coordinately and instead undergoes local vesiculation. Erythrocyte deformation is generally attributed to a dynamic cytoskeleton but the contribution of plasma membrane biophysical properties is not understood. Recent unveiling of stable submicrometric lipid domains at the erythrocyte plasma membrane compelled us to here investigate their contribution to erythrocyte deformation and vesiculation. We used toxin fragments specific to endogenous lipids and fluorescent tools to probe lipid order while developing vital imaging-compatible approaches to address erythrocyte reshaping. We revealed that lipid domains and their biophysical properties contribute with the cytoskeleton to erythrocyte deformation and vesiculation
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Citations
Léonard, C. (2017). Contribution of plasma membrane lipid domains to red blood cell (re)shaping. https://hdl.handle.net/2078.5/177551