Electron-spin-resonance Study of Radiosterilization of Antibiotics - Ceftazidime
(1994) Journal of Pharmaceutical Sciences — Vol. 83, n° 1, p. 68-71 (1994)
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- Abstract
- Fundamental information on radiosterilization of ceftazidime was obtained by electron spin resonance (ESR) measurement of free radicals produced in gamma-ray-irradiated ceftazidime at 4.2-295 K. Three types of free radicals are produced by gamma-irradiation. The first one shows septet lines in an ESR spectrum and decays at 230 K: it is assigned as a .C(CH3)(2)COOH radical. The second one shows triplet lines and decays at 293 K: it is assigned as iminoxyl radicals (>C=N-O.). The third one shows a broad singlet line and survives even at 295 K. The plausible assignment of this spectrum is discussed. It is concluded that an O-C bond of the gamma-irradiated ceftazidime is ruptured, producing the iminoxyl radicals and .C(CH3)(2)COOH radicals. The yields of free radicals increase linearly with the increasing dose of gamma-irradiation up to 10 kGy at 295 K as well as 77 K. Half of the radicals that show a broad singlet line survive at 295 K upon storage of the irradiated ceftazidime for 159 days. It is concluded that the ESR measurement of free radicals gives a good method for discrimination of irradiated antibiotics from non-irradiated ones and an estimation of an irradiation dose.
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Miyazaki, T., Kaneko, T., Yoshimura, T., Crucq, AS., & Tilquin, B. (1994). Electron-spin-resonance Study of Radiosterilization of Antibiotics - Ceftazidime. Journal of Pharmaceutical Sciences, 83(1), 68-71. https://doi.org/10.1002/jps.2600830116 (Original work published 1994)