On-line determination of fluconazole in blood and dermal rat microdialysates by microbore high-performance liquid chromatography.

Mathy, François-Xavier;Vroman, Benoît;Ntivunwa, Denis;De Winne, Ann J;Préat, Véronique;et.al.
(2003) Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences — Vol. 787, n° 2, p. 323-331 (2003)

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Authors
  • Mathy, François-Xavier
    Author
  • Vroman, BenoîtUCLouvain
    Author
  • Ntivunwa, Denis
    Author
  • De Winne, Ann J
    Author
  • Verbeeck, Roger-K.UCLouvain
    Author
  • Préat, VéroniqueUCLouvain
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Abstract
To study the distribution of fluconazole in the dermis of the rat, on-line microdialysis using double-site sampling coupled with a microbore HPLC system was developed. The chromatographic conditions consisted of a mobile phase of 20 mM diammonium phosphate-acetonitrile (75:25, v/v, pH 7.0) pumped through a microbore C(18) column at 40 microl/min. The eluent was monitored with UV detector with UZ flow cell (30 mm path length) at 210 nm. A microbore 10-port pneumatic valve fitted with two loops of 1 microl was used to collect and directly inject microdialysates from jugular and dermal probes. The retention time was 5.8 min for fluconazole and 10.1 min for its fluorinated analog, UK-54373 used as a retrodialysis marker. The assay was precise, with inter- and intra-assay relative standard deviation values of 0.64 and 0.71%, respectively, and with a good linearity (r=0.999) in the range of 0.15-20 microg/ml with only 1 microl injected onto the column. The LOD and LOQ values for fluconazole were 0.100 and 0.150 microg/ml, respectively. The applicability of the method was demonstrated by studying the disposition of fluconazole in blood and dermis following i.v. bolus at a dose of 10 mg/kg.
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Mathy, F.-X., Vroman, B., Ntivunwa, D., De Winne, A. J., Verbeeck, R.-K., & Préat, V. (2003). On-line determination of fluconazole in blood and dermal rat microdialysates by microbore high-performance liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 787(2), 323-331. https://doi.org/10.1016/S1570-0232(02)00961-3 (Original work published 2003)