New signal processing method for multiplex pyrosequencing results analysis

Ambroise, Jérôme;Deccache, Yann;Irenge, Léonid;Gala, Jean-Luc
(2013) Medical Biodefense Conference — Location: Munich, Allemagne (22.October.2013)

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Abstract
Pyrosequencing is a DNA sequencing technology based on the sequencing-by-synthesis principle that has many applications including rapid genotyping of a broad spectrum of bacteria [1, 2]. Despite the increased use of Next Generation Sequencing for studying microbial diversity, pyrosequencing remains a cost-effective solution for genotyping short DNA stretches within bacterial genomes, hence providing rapid genetic assessment of specified microbial antibiotic resistance mechanisms [3,4]. In some cases, molecular typing may require to genotype multiple DNA stretches, each carrying a resistance determinant. Several pyrosequencing primers could be used simultaneously, with overlapping primer-specific pyrosequencing signals as main issue.
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Ambroise, J., Deccache, Y., Irenge, L., & Gala, J.-L. (2013). New signal processing method for multiplex pyrosequencing results analysis. Medical Biodefense Conference, Munich, Allemagne. https://hdl.handle.net/2078.5/46555