In-phase oscillation of global regulons is orchestrated by a pole-specific organizer
(2016) Proceedings of the National academy of sciences of the United States of America — Vol. 113, n° 44, p. 12550-12555 (2016)
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- Authors
Mignolet, Johann 
- Abstract
- Cell fate determination in the asymmetric bacterium Caulobacter crescentus (Caulobacter) is triggered by the localization of the developmental regulator SpmX to the old (stalked) cell pole during the G1→S transition. Although SpmX is required to localize and activate the cell fate-determining kinase DivJ at the stalked pole in Caulobacter, in cousins such as Asticcacaulis, SpmX directs organelle (stalk) positioning and possibly other functions. We define the conserved σ54-dependent transcriptional activator TacA as a global regulator in Caulobacter whose activation by phosphorylation is indirectly down-regulated by SpmX. Using a combination of forward genetics and cytological screening, we uncover a previously uncharacterized and polarized component (SpmY) of the TacA phosphorylation control system, and we show that SpmY function and localization are conserved. Thus, SpmX organizes a site-specific, ancestral, and multifunctional regulatory hub integrating the in-phase oscillation of two global transcriptional regulators, CtrA (the master cell cycle transcriptional regulator A) and TacA, that perform important cell cycle functions.
- Affiliations
UNIGE CMU
Citations
Janakiraman, B., Mignolet, J., Narayanan, S., Viollier, P. H., & Radhakrishnan, S. K. (2016). In-phase oscillation of global regulons is orchestrated by a pole-specific organizer. Proceedings of the National academy of sciences of the United States of America, 113(44), 12550-12555. https://doi.org/10.1073/pnas.1610723113 (Original work published 2016)