Evaluation of the anti-fibrotic properties of adult-derived human liver stem/progenitor cells
(2014)
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- Authors
- Supervisors
- Sokal, Etienne
- Abstract
- Cirrhosis represents a major health issue for which no effective treatment is available so far, except liver transplantation, hardly accessible for patients because of a growing organ shortage. Stem cell therapy is emerging based on their hepatocyte differentiation potential and immunomodulatory properties. The purpose of this thesis is to evaluate the anti-fibrotic properties of Adult Derived Human Liver Stem/progenitor Cells (ADHLSC) both in vitro and in vivo. We firstly established that ADHLSC express and secrete several anti-fibrotic molecules such as HGF, as compared to human hepatic stellate cells (HSC). As HSC activation is a key element in fibrosis induction, we demonstrated that ADHLSC, upon co-culture conditions, inhibit HSC proliferation and collagen secretion. ADHLSC also induce HSC secretion of anti-fibrotic molecules including HGF and MMP. Finally, we demonstrated that ADHLSC transplantation reduces liver fibrosis in an animal model of chemically induced liver fibrosis.
- Affiliations
Citations
Berardis, S. (2014). Evaluation of the anti-fibrotic properties of adult-derived human liver stem/progenitor cells. https://hdl.handle.net/2078.5/195188