Differentiation of Yeast Species Based On Electrophoretic Whole-cell Protein-patterns

Vancanneyt, M.;Pot, B.;Hennebert, Grégoire;Kersters, K.
(1991) Systematic and Applied Microbiology — Vol. 14, n° 1, p. 23-32 (1991)

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  • Vancanneyt, M.
    Author
  • Pot, B.
    Author
  • Hennebert, GrégoireUCLouvain
    Author
  • Kersters, K.
    Author
Abstract
One-dimensional polyacrylamide gel electrophoretic whole-cell protein patterns of 112 isolates of 20 different yeast species, mainly belonging to the genus Candida, were compared by numerical analysis. This technique allowed to evaluate taxonomic relationships at the species level. Qualitative differences between the protein electrophoregrams of different species enabled to confirm anamorph/teleomorph relations and to detect synonymy between species. The high reproducibility allowed to evaluate quantitative differences found at the infraspecific level. Inter- and intrageneric relationships could be deduced after comparison with other taxonomic criteria. The construction of a database of protein fingerprints is therefore feasible and will be a useful tool for routine identification of yeasts.
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Vancanneyt, M., Pot, B., Hennebert, G., & Kersters, K. (1991). Differentiation of Yeast Species Based On Electrophoretic Whole-cell Protein-patterns. Systematic and Applied Microbiology, 14(1), 23-32. https://hdl.handle.net/2078.5/64531 (Original work published 1991)