A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
(2022) Nucleic acids research — Vol. 50, n° 20, p. 11682-11695 (2022)
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- Telomere elongation is coupled with genome replica- tion, raising the question of the repair of short telom- eres in post-mitotic cells. We investigated the fate of a telomere-repeat capped end that mimics a single short telomere in quiescent fission yeast cells. We show that telomerase is able to elongate this single short telomere during quiescence despite the bind- ing of Ku to the proto-telomere. While Taz1 and Rap1 repress telomerase in vegetative cells, both shelterin proteins are required for efficient telomere extension in quiescent cells, underscoring a distinct mode of telomerase control. We further show that Rad3ATR and Tel1ATM are redundantly required for telomere elongation in quiescence through the phosphoryla- tion of Ccq1 and that Rif1 and its associated-PP1 phosphatases negatively regulate telomerase activ- ity by opposing Ccq1 phosphorylation. The distinct mode of telomerase regulation in quiescent fission yeast cells may be relevant to that in human stem and progenitor cells.
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Vaurs, M., Audry, J., Runge, K. W., Géli, V., & Coulon, S. (2022). A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells. Nucleic acids research, 50(20), 11682-11695. https://doi.org/10.1093/nar/gkac986 (Original work published 2022)